Экспериментально изучены и теоретически обоснованы условия формирования наноструктурированного аффинного слоя на поверхности золотого электрода пьезокварцевого гравиметрического сенсора, предназначенного для высокочувствительного и селективного определения в жидких средах следовых концентраций бэта-агониста – рактопамина. Изучены условия получения подложки на основе самоорганизованных монослоев тиолов и силанов, на поверхность которой с помощью бифункциональных реагентов иммобилизованы конъюгаты рактопамина с белковыми молекулами. С учетом величины концентрационной чувствительности, массы и устойчивости распознающего слоя предложен способ модифицирования поверхности золотого электрода пьезокварцевого сенсора, позволяющий распознавать присутствие рактопамина в пробе на уровне 0,05 нг/мл и осуществлять его определение в диапазоне 0,1 -1 нг/мл.
The conditions of the nanostructured affine layer’s formation on the surface of the gold electrode of the piezoelectric gravimetric sensor intended for highly sensitive and selective detection of trace concentration of beta-agonist (ractopamine) in liquid environments have been experimentally studied and theoretically proved. The conditions of creating of a substrate on the basis of the self-organized monolayers of thiols, silanes have been studied. The conjugates of ractopamine with protein molecules were immobilized on the surface of substrate by the activation with bifunctional reagents. The way of modification of a gold electrode’s surface of the piezoelectric sensor was suggested in consideration of the value of concentration sensitivity, weight and stability of the recognizing layer. This way makes it possible to detect the presence of ractopamine in test sample at the level of 0,05 ng/ml and to carry out its definition in the range of 0,1 - 1 ng/ml.
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